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Journal: bioRxiv
Article Title: A high-throughput exonuclease assay based on the fluorescent base analog 2-aminopurine
doi: 10.1101/2022.09.12.507544
Figure Lengend Snippet: Principle of the 2-aminopurine exonuclease assay. (A) Schematic representation of a DNA substrate containing a 2-AP nucleotide positioned at the 3’ end, where its fluorescence is quenched by the neighbouring bases. Upon cleavage by an exonuclease, the 2-AP is released from the DNA and its fluorescence restored. (B) Comparison of the chemical structures of adenine, guanine, and 2-aminopurine.
Article Snippet: Finally, to screen for potential inhibitors of the M. tuberculosis DnaE1 exonuclease we used a
Techniques: Fluorescence, Comparison